Since the publication of our monograph on seed physiology and biochemistry (The Physiology and Biochemistry of Seeds in Relation to Germination, Sprin ger-Verlag, 1978, 1982), it has been suggested to us that a text covering the same subject area would be appropriate. This book is our response. Unlike the previous volumes, however, this text is not intended to be either a critical or a comprehensive account. Instead it is a more generalized consideration of the essential aspects of seed physiology and biochemistry as we see them. It also includes a substantial amount of new and different material. In a work of this sort it is inevitable that some simplifications must be made, but we hope, never theless, that we have presented the most reasonable conspectus of areas of con troversy and uncertainty. In this respect, literature citations have been kept to a minimum and do not interrupt the text; they are placed at the end of each chapter and are intended to be used as a source for further references. We hope that this book will be of value to students and teachers in uni versities, colleges, and other institutes of higher learning whose courses include plant biology. Although it is particularly appropriate for studies of seed biol ogy, it should also find broader applications in general plant physiology, agri culture, and horticulture.
This volume is compiled based on the proceedings of the 5th International Plant Cold Hardiness Seminar, which was held at Oregon State University, Corvallis, Oregon, USA, August 5 to 8, 1996. Participants representing 16 nations and 22 U. S. states attended the seminar. Researchers came from major laboratories around the world involving plant cold hardiness research. The information compiled in this volume represents the state-of the-art research and our understanding of plant cold hardiness in terms of molecular biol ogy, biochemistry, and physiology. The 1996 International Plant Cold Hardiness Seminar was the fifth of the series; it was first held in 1977 at the University of Minnesota, St. Paul, MN, and since then has met every 5 years. The overall goal of this seminar series is to foster the exchange of ideas and research findings among the diverse groups of scientists studying freezing and chilling stresses from a wide variety of perspectives. This is the only international conference focus ing its programs entirely on low temperature stress in plants. In accordance with the tradi tion, the fifth conference focused on freezing and chilling stress of plants and covered various aspects of plant cold hardiness, including molecular genetics, biochemistry, physi ology, and agricultural applications. All contributors to this volume are eminent researchers who have had significant contributions to the knowledge of plant cold hardiness.
The field of eukaryotic DNA repair is enjoying a period of remarkable growth and discovery, fueled by technological advances in molecular biol ogy, protein biochemistry, and genetics. Notahle achievements include the molecular cloning of multiple genes associated with classical human repair disorders, such as xeroderma pigmentosum, Cockayne syndrome, and ataxia telangiectasia; elucidation of the core reaction of nucleotide excision repair (NER); the discovery that certain NER proteins participate not only in repair, but also in transcription; recognition of the crucial role played by mismatch repair processes in maintenance of genome stability and avoidance of cancer; the findings that the tumor suppressor protein p53 is mutated in many types of cancer, and has a key role in directing potentially malignant, genotoxin-dam aged cells towards an apoptotic fate; and the discovery and elaboration of DNA darnage (and replication) checkpoints, which placed repair phenomenol ogy firmly within a cell-cycle context. Of course, much remains to be learned about DNA repair. Tothat end, DNA Repair Protocols: Eukaryotic Systems is about the tools and techniques that have helped propel the DNA repair field into the mainstream of biological research. DNA Repair Protoco/s: Eukaryotic Systems provides detailed, step-by step instructions for studying manifold aspects of the eukaryotic response to genomic injury. The majority of chapters describe methods for analyzing DNA repair processes in mammalian cells. However, many ofthose techniques can be applied with only minor modification to other systems, and vice versa.